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KMID : 1007520210300010129
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Food Science and Biotechnology
2021 Volume.30 No. 1 p.129 ~ p.135
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An optimized procedure for detection of genetically modified DNA in refined vegetable oils
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Duan Yuzhu
Pi Yan
Li Changwen
Jiang Keji
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Abstract
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In this study, the amplifiable DNA from refined vegetable oils was isolated by using commercial DNA extraction kits based on the CTAB method in combination with nucleic acid enrichment, and then the presence of genetically modified (GM) soybean and maize DNA in the oils was traced by PCR. The results showed that the duration and intensity of heating had no significant effect on the DNA stability and concentration in oils for a short period, suggesting that DNA in oils could be stably reserved for a certain time, thus making it possible to trace down refined vegetable oils reliably and effectively. The results provided a set of primers suitable for systematic GM oil detection. More importantly, this study made an important contribution to the economical and reliable detection of GM vegetable oils regarding food authenticity issues.
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KEYWORD
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Refined vegetable oil, PCR, Enrichment treatment, Genetically modified oil detection, DNA isolation
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